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Biocatalytic potential of laccase-like multicopper oxidases from anne frank review essay, Aspergillus niger. © Tamayo-Ramos et al.; licensee BioMed Central Ltd. 2012. Accepted: 19 December 2012. Ap Biology Essay Answer? Published: 27 December 2012. Anne Review? Laccase-like multicopper oxidases have been reported in suns questions several Aspergillus species but they remain uncharacterized. The biocatalytic potential of the Aspergillus niger fungal pigment multicopper oxidases McoA and McoB and ascomycete laccase McoG was investigated.

The laccase-like multicopper oxidases McoA, McoB and McoG from the commonly used cell factory Aspergillus niger were homologously expressed, purified and analyzed for their biocatalytic potential. Book? All three recombinant enzymes were monomers with apparent molecular masses ranging from 80 to 110 kDa. Rules Book Report? McoA and McoG resulted to be blue, whereas McoB was yellow. The newly obtained oxidases displayed strongly different activities towards aromatic compounds and synthetic dyes. McoB exhibited high catalytic efficiency with N,N -dimethyl- p -phenylenediamine (DMPPDA) and 2,2-azino-di(3-ethylbenzthiazoline) sulfonic acid (ABTS), and appeared to be a promising biocatalyst. Besides oxidizing a variety of phenolic compounds, McoB catalyzed successfully the review, decolorization and free to write detoxification of the widely used textile dye malachite green. The A. niger McoA, McoB, and McoG enzymes showed clearly different catalytic properties. Yellow McoB showed broad substrate specificity, catalyzing the oxidation of several phenolic compounds commonly present in different industrial effluents. It also harbored high decolorization and detoxification activity with the synthetic dye malachite green, showing to anne frank have an interesting potential as a new industrial biocatalyst.

Multicopper oxidases (MCOs) form a family of redox enzymes that catalyze the reduction of molecular oxygen into water by a four-electron transfer process. It includes laccases (EC 1.10.3.2), ascorbate oxidases (EC 1.10.3.3), bilirubin oxidases (EC 1.3.3.5) and ferroxidases (EC 1.16.3.1), which are key enzymes in many biological processes of homework florida, prokaryotic and frank review eukaryotic organisms[ 1 , 2 ]. Homework Florida? In fungi, complex MCO gene families exist, possibly due to the variety of frank review, functions they accomplish. Fungal MCOs are involved in delignification, morphogenesis, pigment formation, pathogenesis, competitor interactions and rules report transport of metal ions[ 2 , 3 ]. Their ability to react with a variety of aromatic compounds, by producing just water as a by-product, makes them interesting green biocatalysts[ 2 , 3 ]. As such, they can become key for sustainable industrial processes, like textile production or bioremediation[ 4 , 5 ]. The majority of fungal MCOs are distributed, according to frank essay Hoegger et al .[ 6 ], within the basidiomycete laccases, the ascomycete laccases, the fungal pigment MCOs and the fungal ferroxidases clusters. Laccases form the largest subgroup within the MCO family and they have received most of the attention in biochemical and biotechnological studies[ 6 ]. In particular, basidiomycete laccases of several Trametes and Pleurotus species, amongst others, have been well characterized[ 2 ]. Fungal pigment MCOs, mainly found in a thousand questions ascomycetes[ 7 ], have been reported in several Aspergillus species: A. Anne Book? nidulans LccD, TilA and a beautiful mind essay YA[ 8 – 10 ]; A. fumigatus Abr2[ 11 ]; and A. niger McoA, McoB and McoC[ 12 ]. Although these enzymes are known to oxidize a wide array of substrates[ 8 , 13 ], they have never been characterized. Therefore no information is available about frank essay, their molecular properties or substrate specificities. Aspergillus MCOs included in the ascomycete laccases cluster have also received little attention. A significant number of these enzymes, including: A. nidulans LccA, LccB and LccC[ 8 ]; and A. A Thousand Splendid Essay Questions? niger McoD, McoF, McoG, McoI, McoJ and anne frank book essay McoM[ 12 ], remain uncharacterized.

Interestingly, A. niger MCOs (both, the ones that belong to the fungal pigment MCO cluster, and to the ascomycete laccase cluster) have a low similarity to laccases included in the basidiomycete laccases cluster (around 25% identical). Ap Biology Essay Answer? They also differ from the few deeply characterized ascomycete laccases (i.e. around 25-30% identical to Melanocarpus albomyces laccase, MaL). Thus, to obtain insight into the possible biotechnological potential of frank book review essay, this particular group of MCOs, more knowledge about their catalytic properties is required. The activity patterns observed in plate assays of ten A. Splendid? niger laccase-like MCOs that were recently homologously overexpressed, indicated that remarkable biochemical differences exist between them[ 12 ]. Here we address the anne essay, biocatalytic potential of three A. Ap Biology Essay? niger laccase-like MCOs: two fungal pigment MCOs (McoA and McoB), and essay one MCO belonging to answer the ascomycete laccase subfamily (McoG). Their ability to oxidize an array of aromatic compounds and decolorize different dyes was evaluated. Homologous expression, purification and molecular properties of A. Review Essay? niger MCOs. 110 kDa for McoA, 88 kDa for McoB and. 80 kDa for McoG (Figure 1a ), being in all cases higher than the theoretical expected value ( 64 kDa for McoA, 63 kDa for suns essay, McoB and.

65 kDa for McoG). This difference in size may originate from post-translational protein processing, such as glycosylation. Anne Book? Indeed, analysis of the three amino acid sequences with NetNGlyc 1.0 and GPP Prediction Servers revealed the presence of several potential N-glycosylation sites, being more predominant in McoA (data not shown). Ap Biology? Gel filtration, using a calibrated Superdex 200 column, was performed in order to determine the size and subunit composition of the three enzymes. A single peak was observed for the native form of each MCO, with a relative molecular mass estimated to be: 120 kDa for McoA, 96 kDa for McoB and.

99 kDa for McoG (Figure 1b and 1c ). This result, together with the observations made through SDS-PAGE gel analysis, indicates that the native form of the three enzymes has a monomer conformation. SDS-PAGE (a), elution profiles from a Superdex 200 HR 10/30 column (b) of McoA, McoB and anne review McoG. Reference proteins used to book report calibrate the Superdex 200 HR 10/30 column and anne book review calculate the help florida, McoA, McoB and McoG molecular mass (c) . The gel filtration calibration was performed with the anne frank book, following reference proteins: cytochrome c (12 kDa), myoglobin (18 kDa), ?-chymotrypsin (25 kDa), ovalbumin (43 kDa), bovine serum albumin (68 and 136 kDa), 4-hydroxybenzoate 3-hydroxylase (90 kDa), lipoamide dehydrogenase (102 kDa), phenol 2-hydroxylase (152 kDa), catalase (232 kDa), ferritin (440 kDa) and vanillyl-alcohol oxidase (510 kDa). McoA, McoB and McoG were also included in the plot of K av versus LogM r . Ap Biology Essay? Absorption spectra of McoA and McoB ( 1 mg/mL). Embedded in the graph, McoA and McoB color solutions (10-15 mg/mL) can be observed and compared with 10 mM HEPES buffer (pH 7.0). Steady-state kinetic parameters of McoA, McoB and McoG with DMPPDA and ABTS.

7.5 ? 10 3 ± 6.0 ? 10 2. n.d. = not detectable. Effect of pH and temperature on A. niger MCOs activity. Effect of pH in McoA (a), McoB (b) and McoG (c) activity. The initial rate of anne book essay, enzyme activity was measured with DMPPDA and ABTS substrates in ap biology pH range 2.2 to anne book essay 8.0. Effect of temperature in magazine McoB (red line) and anne book McoG (green dashed line) activity. Ap Biology Answer? The enzyme activity was measured, using ABTS as a substrate, in the range 20 to anne frank review essay 100°C, using 100 mM sodium acetate buffer (pH 5.0). Rules Report? Effect of anne frank book essay, potential inhibitors on answer A. Frank? niger MCOs activity. Effect of metal salts and inhibitors on McoA, McoB and McoG activity. The 100% specific activity of McoA, McoB and McoG was 72.3±1.3 U/mg, 236.1±12.6 U/mg and 57.5±10.6 U/mg, respectively. n.d. = not detectable.

Biocatalytic potential with natural and synthetic substrates. Substrate specificity of McoA, McoB and McoG. On How? 7.1 ? 10 3 ± 1.6 ? 10 2. 13.0 ? 10 3 ± 5.6 ? 10 2. 75.6 ? 10 3 ± 2.3 ? 10 3. 1.7 ? 10 3 ± 4.1 ? 10 2. 3.1 ? 10 3 ± 7.9 ? 10 2. 2.9 ? 10 3 ± 3.1 ? 10 2. 7.0 ? 10 3 ± 4.1 ? 10 2. 4.9 ? 10 3 ± 2.9 ? 10 3. 6.6 ? 10 2 ± 3.2 ? 10 2. 6.1 ? 10 3 ± 4.4 ? 10 2. 4.5 ? 10 3 ± 1.2 ? 10 2. 7.3 ? 10 2 ± 5.2 ? 10 2. 3.9 ? 10 2 ± 1.1 ? 10 2. 3.1 ? 10 3 ± 1.1 ? 10 2. 1.6 ? 10 3 ± 1.1 ? 10 2. Frank Book Essay? n.d. = not detectable. Free On How An Essay? The inability of McoA to oxidize 2,6-dimethoxyphenol (as observed before) nor ABTS is a remarkable result, as a significant number of reported laccases react with these two common substrates[ 24 ]. Nevertheless, as discussed in the “Kinetic parameters” section, other laccases from eukaryotic and anne frank review essay prokaryotic sources are not active with ABTS as well. Also, other laccases have been reported to be inactive with laccase model substrates. For instance, the Agaricus bisporus laccase[ 31 ] is splendid questions, unable to convert syringaldazine and EpoA, from Streptomyces griseus , does not oxidize syringaldazine and guaiacol[ 32 ]. The range of frank book review, substrates oxidized varies from a beautiful mind essay, one laccase to another[ 33 ]. It has been suggested that differences in book essay substrate access to the T1 copper site of ap biology essay, laccases could imply different substrate affinities[ 34 ]. In order to study and understand if this factor could have influence in anne review essay the narrow range of substrate specificity of McoA, the availability of its three-dimensional structure would be desirable. 20%. McoB showed the writing, best decolorizing ability with bromocresol purple (41% of decolorization after 20 h) and in particular with malachite green (83% decolorization after 20 h). Review Essay? Decolorization of different dyes (50 mg/L) by McoA (a), McoB (b) and essay answer McoG (c). Purified enzymes were used to decolorize bromocresol purple, amido black 10B, crystal violet, malachite green, blue dextran and bromothymol blue.

Decolorization of different concentrations (50 mg/L, 100 mg/L, 200 mg/L and 400 mg/L) of malachite green by McoB. The MG decolorization ability of book, McoB, when the a thousand suns questions, dye is present at a final concentration of 50 mg/L, is similar to the one reported recently from a new laccase from Trametes sp. 48424[ 37 ], and better than that of the anne book review essay, laccase from the white-rot fungus Ganoderma lucidum [ 38 ]. Rules Report? The direct decolorization of MG during the review essay, cultivation of several basidiomycete and non-basidiomycete fungi has been also investigated, but the reported efficiencies in similar conditions (e.g. incubation time, MG concentration) were lower than the rules book, ones achieved by means of using the purified enzymes[ 39 – 42 ]. In addition, McoB was also able to oxidize higher concentrations of anne frank book, MG. Similar results, have not been reported for other fungal laccases, although recently the Pseudomonas sp. strain DY1, grown in the presence of the essay answer, dye, has been shown to be a very efficient tool for MG degradation, in concentration ranges from 100 mg/L to 1000 mg/L[ 35 ]. Anne Book Review Essay? MG successful degradation (50 mg/L) by other bacterial species has been also reported[ 43 , 44 ]. However, in none of the cases the magazine writing, enzymes involved in frank book review essay the biodegradation process were identified. The results obtained in magazine the present study suggest that McoB could be used in bioremediation processes of anne frank book essay, this compound. To confirm this, the toxicity of MG transformation compounds should be assessed, as its biodegradation pathway varies depending on the biological treatment, and thus the generated intermediates and final products[ 35 , 38 ]. The fact that decolorization of MG was achieved with no need of on how to write an essay, mediators would mean an additional advantage for the use of McoB, as synthetic mediators may be expensive, toxic, and inhibit the enzyme activity at higher concentrations[ 45 , 46 ]. Nevertheless, natural mediators have also been shown to effectively enhance the anne frank essay, transformation of MG in combination with a fungal laccase[ 38 ]. Fungicide activity of MG and its transformation products. A. niger radial growth in essay complete medium agar plates in the presence of 0.25 mg/L, 0.5 mg/L, 1 mg/L, 2 mg/L and 4 mg/L of malachite green or malachite green decolorized with McoB for 3 h (a); and in the presence of 6 mg/L, 10 mg/L and 20 mg/L of malachite green or malachite green decolorized with McoB for 20 h (b and c). The radial mycelium growth was measured 24 h after inoculation. The picture illustrates the anne frank, appearance of A. niger N593 strain, grown in complete medium in free the presence of different concentrations of MG or DMG, 72 h after inoculation. Frank Review Essay? MG= malachite green; DMG= decolorized malachite green.

The toxicity of free sample on how to write, higher concentrations (6 mg/L, 10 mg/L and 20 mg/L) of frank book essay, MG and DMG, obtained by incubating MG with McoB for 20 h was also tested. As expected, MG completely inhibited A. niger growth, whereas a negative effect of DMG in the radial growth of A. niger was hardly observed (Figure 7b ). Furthermore, the radial growth and the mycelium appeareance 72 h after inoculation in complete medium with DMG was not different to the one observed in the control condition, where no dye was added (Figure 7c ). These results show that the compounds generated after MG decolorization by rules, McoB have a lower toxic effect in A. niger when compared with the frank essay, one produced by the untreated dye. Therefore, McoB could be a good candidate to a thousand essay questions be used in detoxification processes of MG. The ability of McoB to detoxify MG without the need of redox mediators is an advantage over anne essay, other MG detoxification processes reported[ 38 , 46 ]. The different biocatalytic properties of the on how to write, A. niger MCOs determined in this study are remarkable. McoA and frank McoB, showing 56% of sequence identity, are severely divergent in their biochemical features.

The potential applications in rules book report wastewaters pre-treatment processes and dye decolorization, along with the fact that yellow MCOs are far less well characterized than their blue counterparts, makes more challenging to continue with a deeper characterization of McoB. Essay? In this sense, it would be interesting to answer test its suitability for applications related to the food industry, as A. niger is frank book review essay, a safe production organism and many of its enzymes are considered generally recognized as safe (GRAS) by the United States Food and Drug Administration[ 47 ]. Also, the possibility to use industrial A. Essay Answer? niger strains to overproduce the anne essay, native form of McoB could overcome issues related to production yields and homework hotline stability[ 48 ], that can occur during heterologous expression of recombinant proteins, and are less expected in frank homologous expression systems[ 6 ]. A Beautiful Essay? Interestingly, the production yields of this enzyme have been recently optimized, together with those of other A. niger MCOs[ 49 ]. By improving the performance of the glucoamylase expression system, A. niger strains able to accumulate extracellular McoB up to 42% of the anne review essay, total secreted protein were generated[ 49 ]. This study reports the first biochemical characterization of A. niger MCOs. The purified McoA, McoB, and book McoG enzymes showed clearly different substrate specificities. Yellow McoB turned out to be the most efficient biocatalyst, showing broad substrate specificity and anne book review high decolorization activity with the rules report, synthetic dye malachite green. The lower fungicide activity of decolorized MG suggests that McoB can be an interesting biocatalyst for bioremediation processes involving this dye. Strains, media and anne review culture conditions. A. niger N593 strains expressing recombinant McoA, McoB and McoG, were used as cell factories to produce the three enzymes[ 12 ]. Complete medium plates were used for spores preparation and homework help fungicide activity tests. Frank Review Essay? Minimal medium[ 50 ] liquid cultures (containing 50 mM of maltose and 0.1 mM of CuSO 4 ) were used for a beautiful mind essay, MCOs production. Anne Frank Review? Liquid cultures were performed at 30°C and 250 rpm in an orbital shaker, in 2 L erlenmeyer flasks containing 800 mL of hotline, minimal medium inoculated with 1 ? 10 6 spores/mL. Anne? All MCO proteins were purified from filtered supernatants of 24 h cultures. Ammonium sulfate was applied at 100% saturation in 3 L of culture supernatant containing McoA.

After centrifugation, the recovered pellet was dissolved in 50 mL of 20 mM Tris-HCl buffer (pH 7.5). The resulting solution was dialyzed against 20 mM Tris-HCl buffer (pH 7.5) and then concentrated using an Amicon Ultra-15 Centrifugal Filter device. Ap Biology Essay? The resulting solution was applied to a Resource Q 1mL column (GE Healthcare) installed in an Akta purifier FPLC system (GE Healthcare). A linear gradient of NaCl from 0 to 1 M in anne frank book review 20 mM Tris-HCl buffer (pH 7.5) was performed in a total volume of 200 mL, at a flow rate of 1 mL/min. Report? McoB and review essay McoG purification started with a binding step using Streamline Q XL agarose particles (GE Healthcare). Prior to the binding step, 1.5 L of culture supernatants containing McoB and McoG were diluted five times in de-mineralized water. 50 mL of Streamline Q XL resin was added to writing each solution, and stirred during 3 h at 4°C. Proteins were eluted from the resin with 50 mL of anne book, a 1 M NaCl solution in 20 mM Tris-HCl buffer (pH 7.5). McoB and McoG solutions were concentrated in a beautiful mind an Amicon Ultra-15 Centrifugal Filter device and the NaCl concentration was severely reduced through several washing steps with 20 mM Tris-HCl buffer (pH 7.5).

McoB and McoG were further purified on Resource Q, using the same protocol as described for anne frank review essay, McoA. Purified enzymes were stored at -80°C in 10 mM HEPES buffer (pH 7.0). Protein concentration was determined using the Bradford reagent (Bio-Rad) and bovine serum albumin as standard. SDS-PAGE was carried out answer using Precast Polyacrylamide (12%) Mini Gels (Thermo Scientific). Page Blue Protein Stain (Fermentas) was used for SDS-PAGE gels staining. Glycosylation sites in McoA, McoB and McoG amino acid sequences were predicted using NetNGlyc 1.0 ( http://www.cbs.dtu.dk/services/NetNGlyc/ ) and GPP Prediction Servers ( http://comp.chem.nottingham.ac.uk/glyco/index.html ). Analytical gel filtration was performed on a Superdex 200 HR 10/30 column (GE Healthcare), using a similar protocol to the one previously described[ 51 ]. All chemicals were purchased from anne book review, Sigma and Invitrogen. McoA, McoB and McoG activity was determined using similar conditions as previously described[ 12 ], by measuring the homework help hotline, initial oxidation rate ( ? ?), during a period of anne review essay, 6 min, of 6.0 mM ABTS at 420 nm and 2.5 mM DMPPDA at free to write 550 nm (pH 5.0). Enzyme kinetics were determined measuring the oxidation rate of, at least, 10 different solutions of ABTS and essay DMPPDA, in rules book report concentrations ranging from 0.125 to 15 mM. The kinetic parameters of the three enzymes were determined by Michaelis-Menten analysis using the Sigma Plot 8.0 Software for Enzyme Kinetics . V max values were expressed in ? absorbance/min mg protein. Effect of pH and temperature on MCO activity. The pH-dependent activity of McoA, McoB and McoG with ABTS (6.0 mM) or DMPPDA (2.5 mM) was measured in McIlvaine's buffer ranging from pH 2.2 to 8.0 as mentioned in anne the “Enzyme activity determination” section.

The temperature-dependent activity of McoB and McoG was measured from 30 to 100°C by end point determination. Rules Report? Reaction mixtures (in closed microcentrifuge tubes) were incubated at frank different temperatures for 6 min with ABTS (6.0 mM) in 100 mM sodium acetate buffer (pH 5.0). After an incubation step on ice of 5 min, the absorbance of the samples was measured at 420 nm. Effect of inhibitors on MCO activity. The activity of McoA, McoB and McoG with 2.5 mM DMPPDA in 100 mM sodium acetate buffer (pH 5.0) was measured at 23°C in the absence and presence of 1.0 mM NaN 3 ; 1.0 mM SDS; 50 mM EDTA, CaCl 2 , and MgCl 2 ; 100 mM NaCl; different concentrations of CuSO 4 (0.1, 0.5, 1.0 and 2.5 mM) and 50% methanol, ethanol and acetone. The activity of rules book, McoA, McoB and frank book review essay McoG with the to write, following aromatic compounds was investigated: ADBP, phenol, 2,6-dimethoxyphenol, hydroquinone, vanillin, p -coumaric acid, vanillic acid, ferulic acid, syringic acid, and cinnamic acid. Anne Frank Essay? Quantification of substrate specificity was achieved through direct measurement of oxygen consumption. For this purpose an Oxytherm (Hansatech Instruments) was used. All reaction mixtures contained 1 mM of substrate and 100 mM sodium acetate buffer (pH 5.0), and a beautiful reactions were performed at 23°C. One unit of anne frank review essay, enzyme activity (U) was defined as the florida, amount of enzyme that oxidizes 1 ?mol of book review, substrate per min. A Thousand Suns Questions? Bromocresol purple (440 nm), amido black 10B (600 nm), crystal violet (560 nm), malachite green (600 nm), blue dextran (600 nm) and bromothymol blue (440 nm) were incubated with 100 ?g/mL MCO in 100 mM sodium acetate buffer (pH 5.0) at essay 55°C for 20 h. As a negative control, the different dyes were incubated with an equal volume of 10 mM HEPES buffer (pH 7.0).

The degree of dye decolorization was measured 3 and 20 h after the incubation started. Fungicide activity of a beautiful introduction, MG and its transformation products. A malachite green solution (100 mg/L) was incubated with McoB or an equal volume of 10 mM HEPES buffer (pH 7.0), for anne frank essay, 3 and 20 h, following the conditions mentioned in the “Dye decolorization” methods section. After the 3 h incubation, DMG and mind essay introduction MG were mixed with Aspergillus complete medium in 24 well agar plates to reach the following concentrations: 0.25 mg/L, 0.5 mg/L, 1 mg/L, 2 mg/L and 4 mg/L. Similarly, after 20 h incubation, complete medium agar plates were prepared with DMG and MG at the following concentrations: 6 mg/L, 10 mg/L and 20 mg/L. DMG and MG toxicity was measured by frank book review, their ability to inhibit or reduce A. niger mycelium radial growth after an incubation period of sample on how to write, 24 h. In order to monitor toxicity effects and major growth delays produced by DMG or MG, the mycelium growth was monitored for two weeks. We thank Adrie Westphal, Teunie van Herk, Willy van den Berg, Laurens van der Vlist and Martijn Wapenaar for their invaluable assistance. We also thank the reviewers for their valuable comments. This work has been carried out under the anne frank essay, IBOS SME research project 053.63.322 granted by the ACTS national research program from the Netherlands. JATR was recipient of a postdoctoral contract within research project 053.63.322.

Authors' original submitted files for images. The authors declare that there are no competing interests. JATR designed and performed the experimental work and wrote the manuscript. WJHB collaborated in the coordination of the research and helped to draft the manuscript. LG designed the study and helped to a beautiful introduction draft the frank review essay, manuscript.

All authors read and approved the submission of the introduction, manuscript. Quintanar L, Stoj C, Taylor AB, Hart PJ, Kosman DJ, Solomon EI: Shall we dance? How a multicopper oxidase chooses its electron transfer partner. Frank Book Review Essay? Acc Chem Res. 2007, 40: 445-452. 10.1021/ar600051a View ArticleGoogle Scholar Giardina P, Faraco V, Pezzella C, Piscitelli A, Vanhulle S, Sannia G: Laccases: a never-ending story. Cell Mol Life Sci.

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10.1111/j.1574-4976.2005.00010.x View ArticleGoogle Scholar Freeman JC, Nayar PG, Begley TP, Villafranca JJ: Stoichiometry and spectroscopic identity of copper centers in phenoxazinone synthase: a new addition to frank review essay the blue copper oxidase family. Biochemistry. 1993, 32: 4826-4830. A Thousand Splendid Questions? 10.1021/bi00069a018 View ArticleGoogle Scholar Sanchez-Amat A, Solano F: A pluripotent polyphenol oxidase from the melanogenic marine Alteromonas sp shares catalytic capabilities of frank essay, tyrosinases and laccases. Free? Biochem Biophys Res Commun. 1997, 240: 787-792. Anne Frank Review Essay? 10.1006/bbrc.1997.7748 View ArticleGoogle Scholar Galletti GC, Piccaglia R, Chiavari G, Concialini V, Buta JG: Hplc Characterization of Phenolics in Lignocellulosic Materials. Chromatographia. 1988, 26: 191-196. 10.1007/BF02268150.

10.1007/BF02268150 View ArticleGoogle Scholar Akileswaran L, Brock BJ, Cereghino JL, Gold MH: 1, 4-Benzoquinone reductase from Phanerochaete chrysosporium : cDNA cloning and regulation of expression. Appl Environ Microbiol. Magazine Writing? 1999, 65: 415-421. Google Scholar Jaouani A, Guillen F, Penninckx MJ, Martinez AT, Martinez MJ: Role of Pycnoporus coccineus laccase in the degradation of anne frank book essay, aromatic compounds in olive oil mill wastewater. Enzym Microb Technol.

2005, 36: 478-486. 10.1016/j.enzmictec.2004.11.011. 10.1016/j.enzmictec.2004.11.011 View ArticleGoogle Scholar Kunamneni A, Camarero S, Garcia-Burgos C, Plou FJ, Ballesteros A, Alcalde M: Engineering and applications of fungal laccases for organic synthesis. Microb Cell Fact. 2008, 7: 32-, 10.1186/1475-2859-7-32 View ArticleGoogle Scholar Miller R, Kuglin J, Gallagher S, Flurkey WH: A spectrophotometric assay for laccase using o-tolidine. J Food Biochem. 1997, 21: 445-459. Writing? 10.1111/j.1745-4514.1997.tb00199.x. 10.1111/j.1745-4514.1997.tb00199.x View ArticleGoogle Scholar Endo K, Hayashi Y, Hibi T, Hosono K, Beppu T, Ueda K: Enzymological characterization of EpoA, a laccase-like phenol oxidase produced by Streptomyces griseus . J Biochem.

2003, 133: 671-677. 10.1093/jb/mvg086 View ArticleGoogle Scholar Thurston CF: The structure and function of fungal laccases. Microbiology-Sgm. 1994, 140: 19-26. 10.1099/13500872-140-1-19. Anne Review? 10.1099/13500872-140-1-19 View ArticleGoogle Scholar Silva CS, Durao P, Fillat A, Lindley PF, Martins LO, Bento I: Crystal structure of the multicopper oxidase from the pathogenic bacterium Campylobacter jejuni CGUG11284: characterization of ap biology, a metallo-oxidase. Metallomics.

2012, 4: 37-47. 10.1039/c1mt00156f View ArticleGoogle Scholar Du LN, Wang S, Li G, Wang B, Jia XM, Zhao YH, Chen YL: Biodegradation of anne frank book review essay, malachite green by Pseudomonas sp. strain DY1 under aerobic condition: characteristics, degradation products, enzyme analysis and phytotoxicity. Ecotoxicology. Ap Biology Essay Answer? 2011, 20: 438-446. 10.1007/s10646-011-0595-3 View ArticleGoogle Scholar Moumeni O, Hamdaoui O: Intensification of sonochemical degradation of malachite green by anne book, bromide ions. Hotline? Ultrason Sonochem. 2012, 19: 404-409. 10.1016/j.ultsonch.2011.08.008 View ArticleGoogle Scholar Fan F, Zhuo R, Sun S, Wan X, Jiang M, Zhang X, Yang Y: Cloning and functional analysis of a new laccase gene from Trametes sp.

48424 which had the high yield of laccase and strong ability for decolorizing different dyes. Bioresour Technol. 2011, 102: 3126-3137. Frank Book Review? 10.1016/j.biortech.2010.10.079 View ArticleGoogle Scholar Murugesan K, Yang IH, Kim YM, Jeon JR, Chang YS: Enhanced transformation of malachite green by writing, laccase of Ganoderma lucidum in the presence of natural phenolic compounds. Frank Book? Appl Microbiol Biotechnol. 2009, 82: 341-350.

10.1007/s00253-008-1819-1 View ArticleGoogle Scholar Levin L, Papinutti L, Forchiassin F: Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes. Bioresour Technol. 2004, 94: 169-176. 10.1016/j.biortech.2003.12.002 View ArticleGoogle Scholar Yan K, Wang H, Zhang X, Yu H: Bioprocess of homework hotline florida, triphenylmethane dyes decolorization by Pleurotus ostreatus BP under solid-state cultivation. J Microbiol Biotechnol. 2009, 19: 1421-1430. Frank Book Essay? View ArticleGoogle Scholar Ali H, Ahmad W, Haq T: Decolorization and degradation of malachite green by Aspergillus flavus and Alternaria solani . Afr J Biotechnol. 2009, 8: 1574-1576.

Google Scholar Jasinska A, Rozalska S, Bernat P, Paraszkiewicz K, Dlugonski J: Malachite green decolorization by non-basidiomycete filamentous fungi of Penicillium pinophilum and Myrothecium roridum . Int Biodeterior Biodegrad. Magazine? 2012, 73: 33-40. View ArticleGoogle Scholar Parshetti G, Kalme S, Saratale G, Govindwar S: Biodegradation of malachite green by Kocuria rosea MTCC 1532. Acta Chimica Slovenica. 2006, 53: 492-498. Google Scholar Cheriaa J, Khaireddine M, Rouabhia M, Bakhrouf A: Removal of triphenylmethane dyes by bacterial consortium. Frank Review? Sci World J. 2012, 2012: 512454- View ArticleGoogle Scholar Garcia O, Camarero S, Colom JF, Martinez AT, Martinez MJ, Monje R, Vidal T: Optimization of magazine, a laccase-mediator stage for TCF bleaching of flax pulp. Essay? Holzforschung. 2003, 57: 513-519. View ArticleGoogle Scholar Papinutti VL, Forchiassin F: Modification of magazine writing, malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium . FEMS Microbiol Lett. 2004, 231: 205-209.

10.1016/S0378-1097(03)00957-1 View ArticleGoogle Scholar Schuster E, Dunn-Coleman N, Frisvad JC, Van Dijck PW: On the frank book review, safety of Aspergillus niger –a review. Appl Microbiol Biotechnol. Book Report? 2002, 59: 426-435. 10.1007/s00253-002-1032-6 View ArticleGoogle Scholar Vazquez E, Corchero JL, Villaverde A: Post-production protein stability: trouble beyond the cell factory. Microb Cell Fact. 2011, 10: 60-, 10.1186/1475-2859-10-60 View ArticleGoogle Scholar Tamayo-Ramos JA, Barends S, de Lange D, de Jel A, Verhaert R, de Graaff L: Enhanced production of frank book, Aspergillus niger laccase-like multicopper oxidases through mRNA optimization of the glucoamylase expression system.

Biotechnol Bioeng. 2013, 110: 543-551. 10.1002/bit.24723 View ArticleGoogle Scholar van der Veen D, Oliveira JM, van den Berg WA, de Graaff LH: Analysis of variance components reveals the contribution of sample processing to transcript variation. Appl Environ Microbiol. 2009, 75: 2414-2422. 10.1128/AEM.02270-08 View ArticleGoogle Scholar Montersino S, van Berkel WJ: Functional annotation and characterization of 3–hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1. Biochim Biophys Acta. 2012, 1824: 433-442. 10.1016/j.bbapap.2011.12.003 View ArticleGoogle Scholar. Homework Help? This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is anne frank book, properly cited.

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Simple Software for Better Interview Skills. Learn how to land the job with Big Interview’s powerful video tutorials and virtual interview practice software. The Art of Writing a Great Resume Summary Statement. Guest contributor and professional resume writer Kimberly Sarmiento will help you make your resume stand out with a summary statement. In resume writing, you know you need more than just a list of jobs and education, but how to frank begin? Or rather, how should your resume begin? First, nix the objective statement. Free Sample On How To Write! It is frank review essay, almost impossible to write an objective statement without either telling the hiring manager something they already know or focusing on florida what you want to get from a job.

Both are things you should absolutely avoid in a resume. Instead, use a summary statement or what I like to call your “written elevator speech.” Why You Should Use a Resume Summary Statement. Professionals who work with career coaches and those who have done some solid web research have come to understand that they need to master their “Elevator Speech”. This speech is anne review, a short summary of an employee’s value proposition and should be thought of as a “sales pitch” that is ready to go anytime you run into a potential hiring manager or networking contact. A carefully prepared “Elevator Speech” is rules report, customized to the person giving the speech and contains information that makes the professional stand out while still focusing on things the employer wants to know (excels in cutting costs, not excellent deep sea diver). The same holds true for your “Written Elevator Speech” or resume summary statement.

This “speech” is given at the top of your resume — in anne book review essay the prime resume real estate where a recruiter or hiring manager always looks first. The summary statement serves as an help florida, introduction to frank review the reader that seeks to answer the question “Tell me about help hotline, yourself” in just a few lines of text. The resume summary statement will help your resume stand out by: a. Catching the reader’s attention immediately. b. Ensuring a clear understanding of book essay your top selling points at a glance (important when hiring managers are skimming through dozens of resumes at a time and attention spans are short) c. Answer! Putting emphasis on your career highlights and anne frank essay, key strengths in an easy-to-scan format. d. Briefly communicating your professional objective if relevant (if the objective is not obvious) Every resume can benefit from a summary statement. Splendid Essay! For some candidates, it can be critical. 1. Career Changers — A summary statement can help a hiring manager quickly see your transferable skills. Without a summary statement, a recruiter might look at frank book review essay, your most recent experience, assume you’re not a fit because your experience isn’t traditional, and toss your resume.

2. Recent College Grads — A summary statement can help you customize your resume for different opportunities. This is writing, especially helpful if your background is somewhat general. You can use the summary to highlight skills and experience most relevant for frank, each position. 3. Hotline Florida! Experienced Professionals with Diverse Backgrounds — For experienced professionals, a summary statement can become the “executive summary” of your resume, tailored for frank book review, each position. This allows you to pull the most relevant and impressive skills and book, career accomplishments and feature them at the top of your resume. Elements of anne a Strong Resume Summary Statement. The Basics — Your summary statement should consist of a title and a few lines of text. The text can be in paragraph form and/or use bullets.

The summary statement should appear directly below your contact information at the top of the resume. and should reflect a general (or specific) idea of your career goals. Your Title — When working with my clients on their resumes, I typically recommend starting with a title that communicates their professional identity. Think of it as a headline that will catch the sample to write reader’s eye and help them see your fit for anne, the position at hand. Examples include Social Media Brand Strategist, Senior Marketing Executive, Multifaceted Art Director, and Global Operations Professional. See Resume Summary Statement examples below for additional titles that may spark ideas for you. The Format — The main body of your summary statement should be approximately 3-4 lines of text and ap biology answer, should NOT be written with first-person pronouns. If you are tempted to make your summary statement longer to essay squeeze in more details, resist the temptation. Industry research tells us that most hiring managers spend only seconds reviewing a resume before they make up their minds to call a candidate or not. We also know that when they see large chunks of text, their eyes will skip over magazine writing, it.

Therefore, it is vital to limit the length of a summary statement to ensure it gets read. Resume Summary Statement Examples. Below are several examples of “written elevator speeches” or summary/branding statements with titles. A versatile and creative writer fuses a background in journalism and anne book review, academics with expertise in rules book report business writing to deliver quality, customized material spanning news, marketing, web content, curriculum, and career development. Provides sales support and frank book essay, highly-rated client service and excels in essay questions meeting deadlines in book quick-turnaround settings.

FINANCIAL OPERATIONS SUPPORT PROFESSIONAL. Blends academic training in economics and business administration with hands-on experience in sales and operations support to mind essay introduction offer employers a track record of delivering on tasks accurately, efficiently, and quickly. Known for anne frank book review essay, providing best-in-class customer service and communications in ap biology essay answer a variety of anne book essay business settings. A proven leader of US and writing, international sales organizations offers expertise in developing successful growth strategies and training both individual representative and team leaders in product benefits and anne frank review essay, customer service techniques. Also known for creating dynamic marketing/brand strategies that engage consumers and take away business from the competition. Effectively manages PL on multimillion-dollar, multi-product lines of business. How to Write Your Resume Summary Statement. Since you have limited space, it’s important to carefully plan what goes into your summary statement.

Your statement must be concise AND represent the magazine strongest elements of you as a professional. Here are three steps to writing a strong summary statement for your resume: Step 1: First, think of three or four things that define you as a professional. This can be a strong sales record, excellent customer service, expertise in drawing engineering plans, or an ability to manage large-scale technical projects. These professional traits will vary according to profession and skill level.

Managers and executives should focus on business skills as well as technical expertise — even if they fall into a technical industry. Entry-level and anne essay, recent graduates can include academic training and magazine, experience to support professional abilities. Step 2: Next, think of the things you enjoy the most in your work. When you write your summary statement, you aren’t just telling the employer what you are good at, you are also telling them what you want to frank book review essay do day in and day out. Ergo, no matter how well you do something – don’t talk about it if you don’t want to do it. My wisest clients have pointed it out to me when I have focused too much on magazine something they don’t particularly enjoy and anne book essay, we rework their summaries accordingly. Step 3: Align your summary statement with the company’s job requirements. Once you identify the skills you want to focus on, do a little research and see if they line up with job requirements listed for magazine writing, the positions you are seeking.

If you are a project manager, you probably want to frank review essay establish early that you are skilled at a beautiful essay introduction, managing resources and ensuring assignments get completed on-time/on-budget. Anne Frank Book! This might not be the suns questions thing you want to focus on the most, but it is essential to work in. More Tips on Resume Summary Statements. 1. Customize for Your Experience Level. Each of the above example statements effectively sets up the skill sets, achievements, and even certifications/degrees that the applicants highlight throughout their resumes. When writing your statement, it is important to consider where you are in review essay your professional progression. While a job description might want an MBA, PMP, or other certifications, whether or not you mention such things in your opening statement will depend greatly on how much experience you have to magazine back your application. When the job seeker is anne frank review, young and needs to rely on academic experience to strengthen their qualifications, it is best to a beautiful essay introduction call that out from the start. Anne Frank Book Essay! Consider the example below: Blends lab management experience with academic training at the University of on how to write Florida to offer solid skills in anne frank clinical experiments and research activities.

Incorporates a background in office administration to provide employers with proven organization, communications, and scheduling expertise. In this instance, the book report job seeker focused on things learned through education and transferable skills that could be applicable from part-time work experience. On the other hand, when the job applicant has strong experience, there is no need to rely on the academic training any longer and it doesn’t need to be mentioned. 2. Focus on anne frank review your most important selling points. Some requirements can be covered in homework hotline the body of the resume and just aren’t important enough to place in that opening paragraph. One example I see a lot is proficiency in MS Office.

Even if you are a technical professional, software and hardware skills need their own section on book review the resume and don’t belong in the opening statement. The summary statement is for the strengths and accomplishments that truly make you stand out as a candidate. Consider the following examples of resume summaries for technology professionals: A proven leader of IT startups and established operations offers expertise in defining technical strategies that support overall business objectives. Supports efforts to develop and market technical solutions to writing both internal and external clients. Oversees team development and vendor selection/maintenance for multimillion-dollar operations. COMPUTER/HARDWARE SPECIALIST /TEAM SUPERVISOR.

Leverages technical expertise on hardware setup/configuration to provide exceptional user support and resolve critical operational issues. Frank Review Essay! Experience includes managing security and magazine, after-hour support for classified materials and frank review, communications. Known for creating and implementing training that expands team member capabilities and instructs users on writing system utilization/improvement. Works with government, civilian, and frank review essay, corporate stakeholders. Of course there are other things you might want to hotline florida call to the reader’s attention early, including language proficiencies, award-winning performance, or being named on review essay one or more US patents. While these qualifications can be contained in magazine writing the body of a resume (and should still be placed there) , it could be relevant to highlight them early to frank establish your unique value as an employee.

Please note again that none of these opening paragraphs are written with first person pronouns. While you do write the paragraph in present tense, you write it as if you are the understood subject of the resume. This allows the focus to mind remain on the employer. Use of “I, me, my” places the focus on the applicant and the goal of the anne review essay resume is to sell the employer on what you can do for THEM. By telling the reader what you “do” and what you are “known for,” you get the reader thinking about how you can do those things for homework hotline, them. This message should be reinforced throughout the resume as you use achievements and certifications to reinforce your opening paragraph and highlight examples of review essay when you have done the things that summary statement promotes. Remember, aside from a cover letter, the summary statement is the very first thing the hiring manager reads about you. It is your handshake long before you meet the interviewer. It is your first impression long before you get a chance to pick out your best outfit. It requires more than “I am seeking a job as….” It is your first chance to answer the a beautiful mind introduction question “Tell me about Yourself” and you want to make the most of it. HUMOR: Also, if you’re struggling for ideas, why not let Barney Stinson inspire you with some “creative” ways to highlight your awesomeness.

Pamela Skillings is co-founder of Big Interview. As an anne review essay, interview coach, she has helped her clients land dream jobs at companies including Google, Microsoft, Goldman Sachs, and JP Morgan Chase. She also has more than 15 years of experience training and advising managers at organizations from American Express to the City of ap biology essay New York. She is an adjunct professor at New York University and an instructor at the American Management Association. 20 Comment to The Art of Writing a Great Resume Summary Statement. Nice one Pamela. One more thing I would like to add here is one should never try to add any fake information in their resume which can put them in anne frank essay trouble.

@terrylewis – thats great. Maybe you can share it here once you’re done. @Arman – that’s correct. Book Report! Faking info will very likely come back to bite you. So resist the temptation to be too “creative”. What an exceptional post Pamela.

I have seen many articles on book resume tips but nothing like this one. Just want to add more tip here that job seekers should never try to add any fake information in their resumes just to ap biology answer impress the recruiter. This is the part that is frank book, very difficult for me, i work in the service area, i work for a very prestigious hotel brand and i don’t know how to convert what i do into a beautiful introduction a summary. I want to advance in my career, i want to move in anne frank int administrative part of the ap biology hotel, not front office. No I’m not a front desk agent nor a concierge. Book Review Essay! I am having so much trouble coming with the perfect resume so i can apply to mind better jobs.! Here’s a way to possibly get unstuck and draft your summary statement: 1) Brainstorm a bit about the most important qualities required for the job(s) that you want. If there’s a job description, scan it for keywords. 2) Think about your 3 most valuable transferable skills/strengths/areas of book expertise and how they relate. 3) Draft a sentence about each of those three selling points.

4) Combine/arrange/edit them into a summary statement using the examples in the post for book report, inspiration. Depending on your level of experience, your statement may be a few simple sentences or a brief bulleted list. Please feel free to post a draft of your summary statement here and book, I would be happy to a beautiful essay give some feedback. Keep track of the book review interviews. Maintain a record of the .information about the interview so that you can refer to it in future. Many times you would have more than one interview with an organisation and it would help if you have jotted down somewhere what happened in the previous interviews. As the human memory fades with time it is preferable that you record the information within 24 hours of the magazine writing interview. While maintaining the summary of interviews include the anne frank review following information:• Name and position of interviewer(s) After the Interview. It is not over until you finally get the job.

So don’t just sit back and relax. Now is the time to do the follow up procedures to finally get selected and ap biology, gain the frank book essay job of your dreams. Get Selected at Job Interviews. • Job profile/description: duties, responsibilities, powers, salary, other benefits etc. • Category: positive, negative, average. • Date of contact with the organisation. • Specific points discussed: stressed on free sample on how to write some qualifications, achievements more than others. Pamela you are genius…Just read your blog….It is simply amazing. Frank! Already started working on creating my own personal branding statement. It would be great if you can suggest what should be the caption or heading for personal branding statement.

For instance, the categories/headings which appear in a Resume/CV are Core Competencies, Professional Experience, Academics, Personal Information etc. Is it a good idea to write heading ” Senior Banking Professional ” followed by a beautiful essay branding statement under this heading. Frank! Since, I am a banker with 8 years of experience in suns questions executing cross- functional assignments in Retail ( Marketing Campaigns for Retail banking ), Corporate Banking (Commercial Credit to SMEs ) and Strategy (Launch of Wealth Management Business) with MBA as background. Keeping my fingers crossed …to discover a nice branding statement for me… THANKS A LOT…. Outstanding post Pamela! I am a young creative professional and find it very difficult to put my skills and experience into a summary when its such a broad field. After reading your article I now have a better understanding of what companies look for in a resume. Thank you! Outstanding post Pamela and very helpful but I am some time confused that – What type of CV format should I opt for HR post please help as I am working as an anne review essay, HR?

What type of frequent question can be asked during an interview? How can we describe a healthy interview? How can I create my own profession brand as an HR? It seems like the examples used are trying to show some corporate bean counter that you know a lot of big words. Is this really what sells? I don’t want to jump through a bunch of hoops to dazzle some corporate sell out. What ever happend to keeping it simple? Great article. Rules Report! I’m running into trouble highlighting my transferable skills that I got from frank essay being a corporate lawyer because I now want to work on research teams for news shows, or in answer production.

Its difficult trying to review connect the homework help two. Can you write an example here. Good article, exposed some weaknesses in anne frank book essay my resume, I’m make the changes today! Thanks. Helpful article, though I find myself wondering at the images you chose for the sections. They are all part of a larger work by Pablo Picasso, titled “Guernica,” which was his attempt at a propaganda piece criticizing war. The painting depicts innocent people being firebombed by rules book German and anne, Italian warplanes. Clearly shown are tormented soldiers, a dead child in the arms of essay a ravaged woman, and citizens and livestock burning alive. Perhaps this is anne frank, a strange choice of artwork to free sample an essay use for this article. Awesome. shares some other resume design skill in AmoLink resume.

Hope it’s useful to all of anne book review us. I found it odd also. This is why doing arts papers while studying for your business degree is so important. Something incredibly ironic about someone who is an expert in selling yourself plastering her blog post with images from a painting as brutally about suffering as this. I guess whoever pieced this page together thought “oh, it’s art, so it must be sophisticated.” And they say I’m irrational for thinking business people aren’t necessarily very bright. Thank you for the inspiring article! I have been struggling to find a job that fits my professional profile and answer, when I finally find it, I get no attention… at all!

Now, I migh have identified some of my common mistakes. Anyhow, I have a big problem. I am a BA in International Relations and I have no idea how to name my professional title…. Book Essay! Any ideas? I rarely get sidetracked by advertisements or odd artwork in blog posts, mainly because I’m inundated with it. But my concentration was quickly.

diverted to the slightly barbaric images as soon as I could make out an. Pamela Skillings is co-founder of Big Interview. Ap Biology Essay Answer! As one of the country's top interview coaches, she has helped her clients land dream jobs at companies including Google, Microsoft, Goldman Sachs, and frank, JP Morgan Chase. She also has more than 15 years of experience training and advising managers at organizations from American Express to mind the City of New York. She is an frank review essay, adjunct professor at a beautiful mind essay, New York University and an instructor at the American Management Association.

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Everything You Need to frank, Know about homework help hotline florida, Careers in Banking Consulting! In collaboration with student societies at Oxford, The Careers Service is hosting two key events ahead of the onslaught of employer presentations, workshops and fairs to help you navigate careers in ‘the city’. On Tuesday 10 October we’ll be hosting ‘Careers in Consulting: Everything You Need to Know’ from 14.00 to 16.30 at Exams Schools. Hear from 3 very different firms (Strategy, Oliver Wyman and CIL Consulting) to frank book review, learn what a week in the life of a consultant is like and gain key advice to free sample, be on top of anne frank book review your game for competitive applications. Then on Wednesday 11 October from 14.00 to 16.30 at a thousand splendid suns essay questions Exams Schools join us for ‘Careers in Banking: Everything You Need to Know’ where you’ll hear from anne frank book review, a range of hotline firms including HSBC, BNY Mellon, Jefferies and Schroders. They will be giving vital tips on how to find your dream job in finance and illustrating what their day-to-day roles involve. These events are being closely supported by a number of Oxford societies including the Finance Society, the Oxford Student Foundation and book review, The Oxford Strategy Group.

No need to book, spaces are allocated on a ‘first-come, first served’ basis. Career Workshops for Researchers in Michaelmas. Positive steps in career development come from meeting others and learning. Researchers at help hotline florida Oxford can come to any of frank review essay our events, but we also have bespoke opportunities for research Masters students, Research Assistants, DPhils and rules book report, Post-Docs. These are listed below for frank review essay, Michaelmas Term. Also look out for Careers Days, invited speakers and skills sessions run by your department, division or post-doc network. For appointments with Rachel Bray or another Careers Adviser of your choice at the Careers Service on Banbury Road, please book through CareerConnect. Rachel Bray also offers regular appointments for scientists at the JR and Old Road Campus, bookable by phoning reception on 01865 274646. Bookings for workshops/events should be through CareerConnect and if you have any queries please email courses@careers.ox.ac.uk. For: Research Assistants and book report, Masters Students When: Thursday 5 October, 9.00 – 12.30 Where: The Careers Service Booking: This event must be booked. To reserve a place please go to CareerConnect.

In this workshop we will look closely at the value of anne essay doing a doctorate in today’s employment market and for ourselves as people. We will consider our personal motivations for this route, the challenges we may need to overcome to secure a place and how to thrive as a PhD student. There will also be opportunity to reflect on alternative pathways towards professional satisfaction. Homework Help Hotline! Pointers towards further advice and book review, support available here at Oxford and more broadly will be given. This event must be booked. To reserve a place please go to CareerConnect.

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Analyzing Evidence In Self-Reliance Big Idea: Highly scaffolded writing lessons to avoid the wild, wild west of anne essay, writing. My students struggle with writing arguments. In previous lessons, I have engaged students in a process of outlining their ideas beginning with drafting a thesis statement, followed by a thousand splendid questions, drafting topic sentences, followed by selecting textual evidence. Today, I will label this a top-down approach for frank review essay the purpose of introducing students to a bottom-up approach. The main goal is to homework hotline, help students gain an understanding of the conceptual interworking of an essay. The hope is that understanding how an essay works at a conceptual level will help students gain control of the book review essay process. Answer! This activity is highly structured and anne frank review essay scaffolded. I find this necessary at ap biology, this point in the school year.

If I don’t walk them through the anne frank book review process of writing arguments, like I plan to today, I can expect to get unorganized writing that feels like the wild, wild west of a thousand splendid suns essay, writing. Students have already been introduced to and have been expected to know these basic elements of an essay. However, these have not made it to their long-term memory yet so I start class by summarizing them and their relationship. Explaining the relationship is the emphasis of book review essay, this introduction and I point that out to students asking them to make a special place in their memory for free sample on how this information. As shown in anne review, this video, I use a diagram on the board to explain this relationship. I identified this for students as a top-down process and let them know there are other ways of sample on how, approaching an book essay, such as the process we are starting today. Writing! I explain that we are following a bottom-up approach for this assignment, which means we are beginning with the process of essay, gathering textual evidence we are interested in discussing. I give students 6 small post-it notes. I ask them to select 6 powerful sentences/phrases from “Self-Reliance” and write each one on one of the post-its. They soon ask what claims the evidence they are to collect are supposed to support.

I say, “Excellent question!” and explain my intention with this process. If nobody asks that question, I suggest that there is a question they should be asking and would guide students to ask what claims these quotes are going to be supporting. I explain that I am giving them freedom of hotline florida, thought. My intention is to anne book essay, push them into situations where they have to think about their perspective of what they read. If they can get to that point, they are in a good position to make arguments about a text and not just summarize, like they tend to do. I have very good thinkers in my class, but this ability fails to show up in homework help florida, their writing because they have not learned to take control of the writing process and use it to show their thinking. I want to push them in this direction, but we are not ready yet so I do have to give them some direction.

I tell them to consider what Emerson is proposing and the implications on anne review, society. I give them some guiding questions to magazine writing, consider: are Emerson’s claims valid? What do you find convincing? These are meant to help students think of their perspective of anne, this essay and find an angle to approach this writing assignment. The next step is for students to write topic sentences for their evidence.

The instructional focus of this step is to help students’ development of free sample on how to write, a skill they struggle with, verbalizing conceptual ideas. When I assign argumentative writing assignments for students, I often get summaries. To address this, I rely on something I have taught them in a previous lesson, Costa’s Levels of Inquiry. Students know that Costa identified three levels of thinking as follows: level 1 is straight out of the text, level 2 interprets the text, level 3 makes larger connections. We talk about these in my classroom very often because they help us discuss analytical writing and help me keep students away from summarizing when the writing task calls for argumentation.

I have different ways of differentiating level 1 from the other two levels. Today I remind students that level 1 comes straight out the anne frank essay text and it is a thousand essay questions, information they did not make up. I remind them that level 2 and level 3 comes from their mind and frank book essay is their way of making sense of the level 1information they are pulling from the text. I use the diagram on the board to engage students in a discussion about the level of answer, thinking required of frank review, each step. I guide them to identify evidence as level 1 and topic sentences and thesis as level 2 or 3 and write this information on the board. I communicate to students that they are drafting topic sentences for the evidence they selected and that they will help me develop a model using evidence form a text they are all familiar with, Their Eyes Were Watching God , which we read together last semester.

I have selected these eight quotes from the text in advance and have written them on the stickies program in my computer. The quotes I selected can be grouped in a beautiful mind introduction, sets of two or three based on the idea they help communicate. I project them on anne frank book essay, the board for them. I explain to report, students that all these quotes reveal something about one of the characters in the novel, Joe Starks, and I read them aloud. I ask students to turn to book review, the classmates they sit with and discuss which quotes point to the same idea about the character.

I give them a couple of minutes for this. Magazine Writing! I then ask one or two groups to share what they came up with. As they share, I move the stickies on my desk top to group the ones students decided go together. One group lumped stickies number 3, 4, and 1 because they all point to anne book essay, jealousy. I use these to show students the next step, which is to magazine writing, draft a topic sentence for these. For the topic sentence, I create a larger stickie, which is meant to show the fact that a topic sentence is the larger idea that will be supported by the evidence selected, which is now in smaller stickies. Anne Review! I ask students to homework help hotline florida, suggest a possible topic sentence for book these three pieces of evidence. Three different students make suggestions and I write the three sentences on the larger stickie. This offers an opportunity to make the point that a topic sentence should be stated as a concept and I make this point in this video.

At the same time, I am able to show them the difference between a topic sentence that establishes a level 2 idea and one that mainly repeats information from the text. This is a central goal of essay, this lesson. Anne Frank Essay! I let students know that they will engage in this process tomorrow, meaning that they will group their stickies based on the idea they point to essay, and draft a topic sentence. We did not have enough time to select the best topic sentence of the three. We will be doing that at the beginning of the period tomorrow.

That will allow us to anne frank review essay, have a complete model of what is expected of students in their assignment.

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